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KMID : 0380020080230060509
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Korean Journal of Biotechnology and Bioengineering
2008 Volume.23 No. 6 p.509 ~ p.512
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The Hydrolysis of Soybean Oil by Lipase Enzyme Catalyst
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Abstract
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The hydrolysis reaction of soybean oil was conducted experimentally by various source enzymes. The analytical result of hydrolysate of soybean oil showed that the compositions were linoleic acid, olic acid, palmitic acid, and stearic acid in order. The enzymes CR-E and CC-E from Candida rufosa and Candida cylindracea had two hold or more hydrolysis conversions than those of Lipase 16, Novozyme 871, and Lipolase-100L under the same conditions. Therefore CR-E and CC-E were selected for further experiments. These two enzymes had similar ranges of optimun conditions as follows: pH 3-6, 35-45℃, and water to soybean oil ratio of 3.3 or above. They finally got conversions 95% above.
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KEYWORD
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Lipase, hydrolysis, soybean oil, fatty acids
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